Analysis Note

To view the Certificate of Analysis for this product, please visit www.cytiva.com.

Application

GST is a commonly used expression marker in cloning that is also used for purification and detection of fusion Proteins. This highly specific antibody to GST is conjugated to horseradish peroxidase and optimized for use in Western blotting with ECL^™ Detection Reagents.

GST is a commonly used expression marker in cloning that is also used for purification and detection of fusion Proteins. This highly specific antibody to GST is conjugated to horseradish peroxidase and optimized for use in Western blotting with ECL^™ Detection Reagents.

Features and Benefits

Offers the advantages of speed, sensitivity, increased safety, and convenience when used with ECL^™ detection systems. Application tested with ECL^™ and ECL^™ Plus reagents in western blotting for sensitive hard copy results Detection of GST is simplified because no secondary antibody is required The conjugate recognizes multiple epitopes of GST and therefore is not reliant on functional GST for fusion Protein detection. It has also been cross-absorbed to E. coli Proteins so that GST-fusion Proteins can be easily and specifically identified against an E. coli background. Binds with high affinity to GST fusion Proteins. Also suitable for colorimetric detection with diaminobenzidine (DAB) and tetramethylbenzidine (TMB). ECL^™ optimized protocols included.

General description

Anti-GST HRP Conjugate.

Legal Information

ECL is a trademark of Cytiva

GST Gene Fusion VectorsA license for commercial use of GST Gene Fusion Vectors under US patent 5,654,176 and equivalent patents and patent applications in other countries must be obtained from Millipore Corp (formerly Chemicon International Inc).

Storage and Stability

Store at 4 to 30 °C ()